Thermophilus muts
Webb20 juni 2024 · MutS is a component of MMR system (Mismatch Repair system) the role of which is to recognize and remove mispaired and unpaired bases resulting from DNA damage and replication errors. MutS does not recognize mutations, but the disturbed conformation of misaligned nucleotides, also called pre-mutational changes (Jiricny, … Webb1 sep. 2016 · Further, we observed that under the conditions of experiment, MutS from D. radiodurans showed a higher affinity towards mismatched DNA than those two others (Fig. 2 a, b). In the presence of ADP, the specificity of MutS from T. thermophilus strongly increased (Fig. 3 b) to exceed even that of MutS from D. radiodurans (Fig. 2 c).
Thermophilus muts
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Webb28 apr. 1997 · A MutS homologue has recently been identified in the extremely thermophilic bacterium, Thermus thermophilus. Here we describe analysis of the … Webb1 mars 2011 · MutS family proteins are widely distributed in almost all organisms from bacteria to human and play central roles in various DNA transactions such as DNA mismatch repair and recombinational events. The small MutS-related (Smr) domain was originally found in the C-terminal domain of an antirecombination protein, MutS2, a …
Webbthermophilus MutS (ttMutS) was prepared as previously described[4].ADNAfragment-expressingT. thermophilus RecA (ttRecA) was generated by PCR using the T. ther-mophilus HB8genomicDNAasatemplate ... Webb10 sep. 2009 · We previously reported the construction of a plasmid vector carrying mutS-lacZ, a fusion gene designed to obtain a bifunctional chimeric protein consisting of MutS (mismatch binding protein) and β-galactosidase (Sachadyn et al., 2007).The fusion gene was created by in-frame cloning of E. coli lacZ gene into an expression plasmid carrying …
WebbGregory Armstrong, in Comprehensive Natural Products Chemistry, 1999. 2.12.4.3.4 Thermus thermophilus. Thermus thermophilus, an aerobic thermophilic bacterium … Webb1 apr. 2003 · Thermus thermophilus MutS protein hydrolyzed ATP to ADP and Pi, and its activity was maximal at 80°C. The kinetic parameters of the ATPase activity at 65°C were Km = 130 µM and kcat = 0.11 s−1.
WebbThe Tth MutS DNA mismatch protein recognizes heteroduplex DNAs containing mispaired or unpaired bases. This 91.8-kDa protein binds in vitro to heteroduplex DNAs containing …
Webb15 sep. 1998 · MutS protein binds to DNA and specifically recognizes mismatched or small looped out heteroduplex DNA. In order to elucidate its structure-function relationships, the domain structure of Thermus thermophilus MutS protein was studied by performing denaturation experiments and limited proteolysis. denmark provoking the limits of toleranceWebb28 apr. 2011 · T. thermophilus HB8 strains lacking mutM (Δ mutM) and ttha0675 (Δ ttha0675) were constructed by substituting the target gene with the thermostable kanamycin-resistance gene, HTK [34], through homologous recombination as previously described [35], [36]. ffg europe \u0026 americas mag ias gmbhWebb1 mars 2011 · MutS family proteins are widely distributed in almost all organisms from bacteria to human and play central roles in various DNA transactions such as DNA … ffgcx reviewWebb1 mars 2003 · The mutS gene from the thermophilic bacterium Thermus thermophilus was PCR amplified, cloned, and expressed in Escherichia coli. The recombinant MutS protein … ffgc flower show handbookWebbWe previously established that Thermus thermophilus MutS2 (ttMutS2) possesses endonuclease activity. In this study, we report that a Smr-deleted ttMutS2 mutant retains … denmark prince philipWebb15 sep. 1998 · MutS protein binds to DNA and specifically recognizes mismatched or small looped out heteroduplex DNA. In order to elucidate its structure-function relationships, … denmark predicted line upWebb丁香通为您找到25条dna修复论文造假信息，包括dna修复论文造假报价行情，优质供应商，图片，品牌等最新信息，丁香通为买家提供用户服务，诚信保障等服务，批发采购dna修复论文造假,上丁香通。 ffg european \u0026 american holdings gmbh